8R43
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H110N in complex with tri-mannuronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.65 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.060, 38.620, 74.630 |
Unit cell angles | 90.00, 116.76, 90.00 |
Refinement procedure
Resolution | 27.700 - 0.870 |
R-factor | 0.12 |
Rwork | 0.120 |
R-free | 0.14090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_4788) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.700 | 0.900 |
High resolution limit [Å] | 0.870 | 0.870 |
Number of reflections | 159325 | 15618 |
<I/σ(I)> | 14.39 | |
Completeness [%] | 99.3 | |
Redundancy | 6.8 | |
CC(1/2) | 1.000 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 30% PEG3350, 0.1M Bis-Tris pH 5.5, 0.2M NaCl |