8R3P

Transketolase from Enterococcus faecium in complex with thiamin pyrophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	249.660, 68.220, 165.460
Unit cell angles	90.00, 110.71, 90.00

Refinement procedure

Resolution	34.940 - 2.900
R-factor	0.2094
Rwork	0.208
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.804
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.940	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	57466	5558
<I/σ(I)>	6.41
Completeness [%]	98.4
Redundancy	2.76
CC(1/2)	0.986	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M MgCl2 0.1 M Tris pH 8.5, 16 % (w/v) PEG 4000