8R2C

Crystal structure of the Vint domain from Tetrahymena thermophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	35.890, 46.360, 115.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 1.800
R-factor	0.1898
Rwork	0.189
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.292
Data reduction software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.864
High resolution limit [Å]	1.790	1.800
Rmerge	0.016	0.111
Number of reflections	18445	1737
<I/σ(I)>	11.3
Completeness [%]	97.1
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	100 nL concentrated protein (14.6 mg/mL) with 100 nL mother liquid (2% PEG 400, 2.4 M ammonium sulfate, 0.1 M HEPES)