8R1X
Solution structure and chemical shift assignments for HMG-D Y12F mutant complexed to a 14:12 dA2 bulge DNA
Replaces: 1E7JSpectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 500 | |
| 2 | Bruker | AVANCE | 800 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 1 | 1 | 3D HCCH-COSY |
| 11 | 1 | 1 | 3D HCCH-TOCSY |
| 12 | 1 | 1 | 3D 1H-15N NOESY (mixing time = 150 ms) |
| 13 | 1 | 1 | 3D 1H-13C NOESY aliphatic (mixing time = 150 ms) |
| 14 | 1 | 1 | 3D 1H-13C NOESY aromatic (mixing time = 150 ms) |
| 15 | 1 | 2 | 2D [13C,15N]-filtered [1H-1H] NOESY (mixing time = 100 ms, with filter elements set to reject 13C and 15N coupled signals in F1 and to accept only 13C coupled signals in F2) |
| 16 | 1 | 2 | 2D [13C,15N]-filtered [1H-1H] NOESY (mixing time = 200 ms, with filter elements set to reject 13C and 15N coupled signals in F1 and to accept only 13C coupled signals in F2) |
| 17 | 1 | 2 | 13C-filtered 13C NOESY-HSQC (mixing time = 150 ms, with filter elements set to reject 13C- and 15N-coupled (protein) signals in F1) |
| 2 | 1 | 1 | 2D 1H-13C HSQC |
| 3 | 1 | 1 | 2D 1H-1H NOESY (mixing time = 60 ms) |
| 4 | 1 | 1 | 2D 1H-1H NOESY (mixing time = 150 ms) |
| 5 | 1 | 1 | 3D HNCA |
| 6 | 1 | 1 | 3D HN(CO)CA |
| 7 | 1 | 1 | 3D HNCACB |
| 8 | 1 | 1 | 3D HBHANH |
| 9 | 1 | 1 | 3D HBHA(CO)NH |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6 | 1 | 300 |
Conformers
| Conformers Calculated Total Number | 60 |
| Conformers Submitted Total Number | 20 |
