8R07
C-terminal Rel-homology Domain of NFAT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-05 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.541800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.456, 40.320, 54.637 |
| Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
| Resolution | 36.180 - 1.740 |
| R-factor | 0.1486 |
| Rwork | 0.147 |
| R-free | 0.18390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.604 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.180 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 19383 | 1358 |
| <I/σ(I)> | 16.82 | |
| Completeness [%] | 88.5 | |
| Redundancy | 2.8 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 25% PEG 3350, 200mM Sodium Chloride and 100 mM BIS-TRIS buffer at a pH of 6.5 |
