8QZY
Structural and biochemical characterization of diaminopimelate epimerase from Pseudomonas aeruginosa
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.542, 88.580, 97.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.580 - 1.690 |
| Rwork | 0.177 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.143 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.450 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.133 | 1.531 |
| Rmeas | 0.139 | 1.592 |
| Rpim | 0.039 | 0.433 |
| Number of reflections | 61204 | 8827 |
| <I/σ(I)> | 14.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 13.2 |
| CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.49 M NaH2PO4, 0.91 M K2HPO4 |
