8QZO

Crystal structure of heterodimeric complex of CdpB1 and CdpB2 from A. fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	71.086, 102.633, 112.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.490 - 2.290
R-factor	0.2337
Rwork	0.231
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	AutoProcess
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.760	2.430
High resolution limit [Å]	2.290	2.290
Rmeas	0.088	1.618
Number of reflections	30565	1527
<I/σ(I)>	11.3
Completeness [%]	89.0
Redundancy	6
CC(1/2)	0.999	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	MPD 5% v/v, magnesium chloride hexahydrate 0.04M, sodium cacodylate trihydrate 0.05M pH 6