8QZI
Crystal structure of PptT-ACP from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.335, 280.843, 47.340 |
Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
Resolution | 47.280 - 2.500 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.24090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.036 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.280 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 42194 | 4122 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 99.6 | |
Redundancy | 3.7 | |
CC(1/2) | 0.990 | 0.555 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.25 | 285 | 0.1 M Hepes 5 % isopropanol 24 % PEG4000 |