8QZA
D-2-hydroxyacid dehydrogenase (D2-HDH) from Haloferax mediterranei apo-enzyme (2.25 A resolution)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.352, 75.986, 74.589 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 29.450 - 2.252 |
| Rwork | 0.190 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.939 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.041 | 0.420 |
| Number of reflections | 32656 | 2669 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 98.8 | 88 |
| Redundancy | 6.8 | 5.1 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | Protein Buffer: 20mM Tris/HCl pH 8.0 2mM EDTA 1M NaCl Crystallization condition: 0.1 M TRIS/HCl pH 8.0 0.5 M Magnesium acetate 20% PEG 3350 |
