8QYB

J22.9-ISY, fully humanized and CDR optimized Fab Fragment based on chimeric J22.9-xi IgG against BCMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	137.185, 54.617, 81.084
Unit cell angles	90.00, 121.09, 90.00

Refinement procedure

Resolution	45.250 - 3.090
R-factor	0.2349
Rwork	0.232
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1-5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.250	3.205
High resolution limit [Å]	3.090	3.090
Rmeas	0.250	0.996
Number of reflections	9514	1380
<I/σ(I)>	5.15
Completeness [%]	99.0
Redundancy	3.6
CC(1/2)	0.975	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	14% PEG 3350 7.5 mM CuCl2 BisTris