8QXW

HCMV DNA polymerase processivity factor UL44 unphosphorylated NLS 410-433 bound to mouse importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	78.853, 90.062, 96.051
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.330 - 2.000
R-factor	0.1927
Rwork	0.191
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.136
Data reduction software	DIALS
Data scaling software	Aimless
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.750	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.098	1.730
Number of reflections	47035	3420
<I/σ(I)>	9.8	0.9
Completeness [%]	100.0	100
Redundancy	8.6	8.2
CC(1/2)	0.998	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	Sodium citrate 0.65M, HEPES pH7.5, DTT 0.01M