8QX7

Apo-C-Terminal Domain Homolog of the Orange Carotenoid Protein from Anabaena at a resolution of 1.95 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	111.929, 52.925, 48.595
Unit cell angles	90.00, 96.88, 90.00

Refinement procedure

Resolution	47.780 - 1.950
R-factor	0.1974
Rwork	0.195
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.780	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.076
Rmeas	0.091
Rpim	0.049
Number of reflections	20695	1410
<I/σ(I)>	12.8
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.998	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	44% Tacsimate pH 7.0