8QX6

Novel laminarin-binding CBM X584

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	55.439, 58.119, 91.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.420 - 1.900
R-factor	0.232
Rwork	0.230
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.148	1.810
Rpim	0.043	0.590
Number of reflections	23977	1610
<I/σ(I)>	10.9	1.3
Completeness [%]	99.8	96.9
Redundancy	12.9	10.2
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	7	292	2.5 M ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0, 6% ethanol and 4% polyethylenglycol (PEG) 6000