8QX5

Helical Carotenoid Protein 4 (HCP4) from Anabaena with bound Canthaxanthin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 41 2 2
Unit cell lengths	59.468, 59.468, 183.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.730 - 1.900
Rwork	0.194
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.730	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.089
Rmeas	0.092
Rpim	0.024
Number of reflections	27148	1679
<I/σ(I)>	25
Completeness [%]	99.9
Redundancy	26.2
CC(1/2)	1.000	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.095M sodium citrate tribasic dihydrate pH=5.6, 19% v/v 2-propanol, 19% w/v PEG 4000, 5% glycerol