8QWX
Ligninolytic manganese peroxidase Ape-MnP1 from Agaricales mushrooms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.623, 39.630, 63.493 |
| Unit cell angles | 90.00, 101.23, 90.00 |
Refinement procedure
| Resolution | 48.781 - 1.600 |
| Rwork | 0.151 |
| R-free | 0.18640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.876 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.781 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.475 |
| Rmeas | 0.061 | 0.569 |
| Rpim | 0.041 | 0.428 |
| Number of reflections | 39894 | 1967 |
| <I/σ(I)> | 14.1 | 2.4 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 30% P2000 MME, 0,1 M sodium citrate, pH 4.2 |
