8QWJ
Structure of GFP variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 37.894, 68.275, 112.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.480 - 1.500 |
| R-factor | 0.2395 |
| Rwork | 0.238 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.202 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.480 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.059 | 1.200 |
| Number of reflections | 47763 | 2365 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.800 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Fusion screen from Molecular Dimension, condition E10 30% PEG 20K/PEG 500 MME (1:2), 0.1 M Bicine/Trizma pH 8.5, 1.5% Morpheus III Vitamins , 1.2% (w/v) Morpheus III Cholic acids |
