8QV4
Hexameric HIV-1 CA in complex with DDD01728503
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-02 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 6 |
Unit cell lengths | 90.399, 90.399, 56.654 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.330 - 2.700 |
R-factor | 0.23305 |
Rwork | 0.230 |
R-free | 0.30264 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.330 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.300 | 1.000 |
Number of reflections | 7164 | 1046 |
<I/σ(I)> | 4.8 | 1.9 |
Completeness [%] | 97.7 | 98.8 |
Redundancy | 6 | 6.1 |
CC(1/2) | 0.960 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.1M Tris buffer, pH 8.0 to 9.0, 10-15% PEG550MME, 0.15M KSCN |