8QV1
Hexameric HIV-1 CA in complex with DDD01728505
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.345, 90.345, 56.581 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.330 - 2.200 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.170 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.290 | 1.330 |
| Number of reflections | 13511 | 1977 |
| <I/σ(I)> | 5.5 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
| CC(1/2) | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.1M Tris buffer, pH 8.0 to 9.0, 10-15% PEG550MME, 0.15M KSCN |
