8QUX

Hexameric HIV-1 CA in complex with DDD00100333

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-02
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 6
Unit cell lengths	90.392, 90.392, 56.618
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.330 - 2.300
R-factor	0.20478
Rwork	0.201
R-free	0.27553
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.435
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.330	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.220	0.840
Number of reflections	11849	1717
<I/σ(I)>	7.1	2.5
Completeness [%]	99.9	100
Redundancy	6.1	6.1
CC(1/2)	0.980	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.1M Tris buffer, pH 8.0 to 9.0, 10-15% PEG550MME, 0.15M KSCN