8QUA
GTP binding protein YsxC from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-08-08 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.942, 55.942, 107.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.046 - 2.000 |
| Rwork | 0.257 |
| R-free | 0.31650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.058 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.050 | 20.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.049 |
| Rmeas | 0.098 | 0.054 |
| Rpim | 0.044 | 0.021 |
| Number of reflections | 13613 | 13613 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.7 | 8.3 |
| CC(1/2) | 0.998 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295.15 | 2.6M Potassium/sodium phosphate pH 7.6 |
