8QU6
Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD and an upstream-fork promoter fragment
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.45 |
| Software | Topaz,RELION,SerialEM,PHENIX |
| CTF correction | PHASE FLIPPING ONLY |
