8QU2

NF-YB/C Heterodimer in Complex with a 16-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	45.080, 51.460, 72.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 1.450
R-factor	0.142
Rwork	0.140
R-free	0.17190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.053	0.746
Rmeas	0.055	0.777
Rpim	0.015	0.215
Number of reflections	30704	3024
<I/σ(I)>	25.42	2.89
Completeness [%]	99.9	99.83
Redundancy	13.2	12.9
CC(1/2)	1.000	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 mM Morpheus Buffer system 3 pH 9.0, 30% (v/v) Morpheus Precipitant mix 1, 100 mM Morpheus Carboxylic acids.