8QTT

Crystal structure of a C-terminally truncated version of Arabidopsis thaliana 14-3-3 omega in complex with a phosphopeptide from the inhibitor protein BKI1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003353518
Spacegroup name	P 1
Unit cell lengths	71.216, 71.082, 150.986
Unit cell angles	100.47, 95.53, 88.74

Refinement procedure

Resolution	50.330 - 2.350
R-factor	0.2365
Rwork	0.234
R-free	0.27550
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.406
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.330	2.490
High resolution limit [Å]	2.350	2.350
Rmeas	0.217	1.856
Number of reflections	118395	18838
<I/σ(I)>	5.5	0.95
Completeness [%]	98.1	96.5
Redundancy	3.7	3.7
CC(1/2)	1.000	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02M of carboxylic acid, 0.1M MOPS/HEPES-Na pH7.5