8QTF

Crystal structure of a C-terminally truncated version of Arabidopsis thaliana 14-3-3 omega in complex with a phosphopeptide from the transcription factor BZR1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999995
Spacegroup name	P 1
Unit cell lengths	71.641, 71.917, 151.662
Unit cell angles	100.25, 94.84, 89.23

Refinement procedure

Resolution	59.910 - 1.900
R-factor	0.1968
Rwork	0.195
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.910	2.020
High resolution limit [Å]	1.900	1.900
Rmeas	0.095	2.110
Number of reflections	227664	36285
<I/σ(I)>	9.47	0.75
Completeness [%]	97.5	96.2
Redundancy	3.2	3.4
CC(1/2)	1.000	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Morpheus (Molecular Dimensions) condition G8 with a final precipitant stock concentration of 50% v/v 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD ,0.02M of each carboxylic acid, 0.1M MOPS/HEPES-Na pH7.5