8QTC
Crystal structure of Arabidopsis thaliana 14-3-3 omega in complex with a phosphopeptide from the transcription factor BZR1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-06 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999999406361 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 131.503, 131.503, 256.357 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 68.350 - 3.500 |
R-factor | 0.2184 |
Rwork | 0.217 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.350 | 3.710 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmeas | 0.339 | 5.050 |
Number of reflections | 17253 | 2692 |
<I/σ(I)> | 14.32 | 0.91 |
Completeness [%] | 99.9 | |
Redundancy | 38.3 | 39.6 |
CC(1/2) | 1.000 | 0.370 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2 M (NH4)2SO4 , 10 mM CoCl2 6 H2O, 0.1 M Mes (pH 6.5) |