8QTA
Cryo-EM structure of Streptococcus pneumoniae NADPH oxidase F397A mutant in complex with NADPH
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.64 |
| Number of Particle | 13817533 |
| Software | Topaz,PHENIX,Coot,cryoSPARC,crYOLO,EPU,CTFFIND,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
