8QT8
Crystal structure of human Sirt2 in complex with a TNFa-Myr analogue TNFn-34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9188 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.106, 73.339, 55.229 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.650 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.084 | 0.837 |
| Rmeas | 0.091 | 0.904 |
| Rpim | 0.035 | 0.339 |
| Total number of observations | 232753 | |
| Number of reflections | 34505 | 1718 |
| <I/σ(I)> | 12.9 | 2.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.998 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 19 % (w/v) PEG 3,350 in 0.1 M Bis-Tris pH 6.7 |
