8QT5

Crystal structure of Arabidopsis thaliana 14-3-3 isoform lambda in complex with a phosphopeptide from the transcription factor BZR1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03320713866
Spacegroup name	P 31 2 1
Unit cell lengths	176.052, 176.052, 172.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.920 - 2.690
R-factor	0.2417
Rwork	0.241
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.406
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.920	2.850
High resolution limit [Å]	2.690	2.690
Rmeas	0.111	3.037
Number of reflections	84698	13110
<I/σ(I)>	19.54	1.09
Completeness [%]	98.5	95.2
Redundancy	20.2	20.8
CC(1/2)	1.000	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	22 % [w/v] PEG 10,000, 0.1 M ammonium acetate, 0.1 M Bis-Tris [pH 5.5]