8QRD

OleP in complex with testosterone in high salt crystallization conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0002
Spacegroup name	C 1 2 1
Unit cell lengths	248.195, 109.738, 160.130
Unit cell angles	90.00, 129.41, 90.00

Refinement procedure

Resolution	45.750 - 2.300
R-factor	0.16762
Rwork	0.165
R-free	0.21226
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.692
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.070	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.108	1.785
Number of reflections	147079	7268
<I/σ(I)>	13.3
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.999	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	4.4 M sodium formate