8QQF

TBC1D23 PH domain complexed with STX16 TLY motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.997
Spacegroup name	P 65 2 2
Unit cell lengths	134.159, 134.159, 123.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.080 - 2.190
R-factor	0.2163
Rwork	0.214
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.971
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (dev_svn+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	2.480
High resolution limit [Å]	2.190	2.190
Rmerge	0.133
Number of reflections	14895	745
<I/σ(I)>	23
Completeness [%]	94.8	100
Redundancy	32.7	82.9
CC(1/2)		0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.8M Potassium phosphate dibasic, 0.1M HEPES/NaOH pH 7.5, 0.8M Sodium phosphate monobasic and 1% 1,2-Butandiol