8QOY
Capsular polysaccharide synthesis multienzyme of Actinobacillus Pleuropneumoniae serotype 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.67 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 207.911, 207.911, 93.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.550 - 3.000 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.489 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.550 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 46791 | 4630 |
| <I/σ(I)> | 9.23 | |
| Completeness [%] | 99.9 | |
| Redundancy | 62.5 | |
| CC(1/2) | 0.998 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 1.6 M magnesium sulphate heptahydrate, 0.1 M MES pH 6.5 |
