8QNN
Crystal structure of a Class A beta-lactamase from Nocardia cyriacigeorgica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.180, 54.180, 131.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.950 - 1.250 |
| R-factor | 0.1426 |
| Rwork | 0.142 |
| R-free | 0.17450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.950 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmeas | 0.078 | |
| Number of reflections | 63063 | 27178 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.8 |
| CC(1/2) | 0.990 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M citric acid pH3.5, 10 % PEG 6000 and 6% ethylene glycol |
