8QNK
OPR3 variant R283D in complex with NADPH4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.576, 90.483, 114.632 |
| Unit cell angles | 90.00, 110.02, 90.00 |
Refinement procedure
| Resolution | 35.900 - 1.910 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (0.88560) |
| Refinement software | PHENIX ((dev_4761)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 1.930 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 114489 | 3915 |
| <I/σ(I)> | 8.56 | |
| Completeness [%] | 95.5 | |
| Redundancy | 4 | |
| CC(1/2) | 0.998 | 0.417 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM Tris-HCl, 50 mM sodium tartrate, 10% PEG8000 |
