8QND
Crystal structure of the ribonucleoside hydrolase C from Lactobacillus reuteri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-18 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.112, 81.530, 86.847 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 63.380 - 1.900 |
| R-factor | 0.16996 |
| Rwork | 0.168 |
| R-free | 0.20077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.835 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.380 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.347 |
| Rmeas | 0.091 | 0.377 |
| Rpim | 0.039 | 0.146 |
| Total number of observations | 585566 | 29786 |
| Number of reflections | 91739 | 4547 |
| <I/σ(I)> | 19.5 | 5.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.4 | 6.6 |
| CC(1/2) | 0.914 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8,000 |
