8QMY
Crystal structure of ancestral L-galactono-1,4-lactone dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.047, 98.016, 238.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.050 - 1.900 |
| R-factor | 0.19655 |
| Rwork | 0.195 |
| R-free | 0.23241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 1.734 |
| Rmeas | 0.100 | 1.873 |
| Rpim | 0.038 | 0.702 |
| Total number of observations | 42710 | |
| Number of reflections | 126385 | 6164 |
| <I/σ(I)> | 9.9 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.998 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20 % PEG 3350, 0.2 M Lithium Nitrate |
