8QMU

The complex of Glycogen Phosphorylase with (-)-Epigallocatechin-3-gallate (EGCG).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	126.855, 126.855, 115.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.700 - 2.000
R-factor	0.16608
Rwork	0.164
R-free	0.20838
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.543
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.860	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	64044	4451
<I/σ(I)>	12	5.3
Completeness [%]	100.0	99.9
Redundancy	10.9	11.4
CC(1/2)	0.994	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.7	289	10 mM BES pH 6.7