8QM8
Potential drug binding sites for translation initiation factor eIF4E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-18 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.418, 42.035, 64.728 |
| Unit cell angles | 90.00, 90.01, 117.22 |
Refinement procedure
| Resolution | 64.730 - 1.585 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20170 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.730 | 1.590 |
| High resolution limit [Å] | 1.585 | 1.585 |
| Rmeas | 0.060 | 1.155 |
| Number of reflections | 36074 | 23 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 70.6 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% PEG 3350 0.1M pH=8 Bicine/NaOH |
