8QM6
Potential drug binding sites for translation initiation factor eIF4E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.293, 69.344, 64.771 |
| Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
| Resolution | 47.190 - 1.930 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22670 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmeas | 0.051 | 0.916 |
| Number of reflections | 31841 | 1706 |
| <I/σ(I)> | 14.2 | 1.5 |
| Completeness [%] | 98.7 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.002M DTT 40% PEG 400 0.1M pH=8 Tris/HCl |
