8QLN

Crystal structure of an N-terminal fragment of HCMV UL47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 43 21 2
Unit cell lengths	64.960, 64.960, 265.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 2.510
R-factor	0.2164
Rwork	0.214
R-free	0.25110
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.380
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.460	2.600
High resolution limit [Å]	2.510	2.510
Rmeas	0.109	1.043
Number of reflections	20390	1930
<I/σ(I)>	18.35
Completeness [%]	100.0	100
Redundancy	25.3	27
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.1 M Sodium acetate pH 4.6, 2 M sodium formate, 5 mM TEW