8QLF

Crystal structure of the light-driven sodium pump ErNaR in the monomeric form at pH 8.8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 61 2 2
Unit cell lengths	53.382, 53.382, 364.842
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.710
R-factor	0.18443
Rwork	0.183
R-free	0.21819
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.230	1.875
High resolution limit [Å]	1.707	1.707
Rpim	0.038	0.518
Number of reflections	26414	1321
<I/σ(I)>	13.769	1.59
Completeness [%]	94.7
Redundancy	37.6
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8.8	293	0.1M sodium acetate, 10% PEG550MME, soaked in 0.1M Tris-HCl pH 8.8, 10% PEG550MME prior to crystal harvesting.