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8QLE

Crystal structure of the light-driven sodium pump ErNaR in the monomeric form at pH 4.6

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP14 (MX2)
Temperature [K]100
Detector technologyPIXEL
Collection date2021-03-29
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.976
Spacegroup nameP 61 2 2
Unit cell lengths53.401, 53.401, 365.052
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution20.000 - 1.700
R-factor0.21354
Rwork0.212
R-free0.24781
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.002
RMSD bond angle1.025
Data reduction softwareXDS
Data scaling softwareSTARANISO
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]60.8421.763
High resolution limit [Å]1.6641.664
Rpim0.0521.161
Number of reflections301151507
<I/σ(I)>11.0380.698
Completeness [%]94.278.4
Redundancy35.9
CC(1/2)0.9990.357
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1LIPIDIC CUBIC PHASE4.62930.1M sodium acetate pH 4.6, 10% PEG 550MME.

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