8QLE
Crystal structure of the light-driven sodium pump ErNaR in the monomeric form at pH 4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 53.401, 53.401, 365.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.21354 |
Rwork | 0.212 |
R-free | 0.24781 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.842 | 1.763 |
High resolution limit [Å] | 1.664 | 1.664 |
Rpim | 0.052 | 1.161 |
Number of reflections | 30115 | 1507 |
<I/σ(I)> | 11.038 | 0.698 |
Completeness [%] | 94.2 | 78.4 |
Redundancy | 35.9 | |
CC(1/2) | 0.999 | 0.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.6 | 293 | 0.1M sodium acetate pH 4.6, 10% PEG 550MME. |