8QKM
Symmetric structure of Satellite Tobacco Necrosis Virus-Like Particle with PS1-5 gRNA
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.39 |
| Number of Particle | 502684 |
| Software | RELION,Coot,cryoSPARC,EPU,Gctf,UCSF Chimera,PHENIX |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
