8QKF
SmtB protein of Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.292, 72.479, 82.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.110 - 1.880 |
| R-factor | 0.19642 |
| Rwork | 0.194 |
| R-free | 0.24351 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.110 | 1.990 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.066 | |
| Rmeas | 0.073 | |
| Number of reflections | 21130 | 3401 |
| <I/σ(I)> | 13.25 | |
| Completeness [%] | 99.3 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.248 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | L-Proline, PEG 3350, HEPES |
