8QK8
Structure of Legionella pneumophila Lcl C-terminal domain bound to sulphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 74.910, 74.910, 94.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.874 - 1.900 |
Rwork | 0.160 |
R-free | 0.18740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.462 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.910 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.074 | 0.541 |
Number of reflections | 24856 | 1235 |
<I/σ(I)> | 28.7 | 5.8 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 19.3 | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (v/v) glycerol, 20% (w/v) polyethylene glycol (PEG) 4000, 30 mM NaNO3, 30 mM Na2HPO4, 30 mM (NH4)2SO4, 100 mM bicine, 100 mM tris-HCl |