8QJS

VHL/Elongin B/Elongin C complex with compound 155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9121
Spacegroup name	P 41 2 2
Unit cell lengths	93.235, 93.235, 361.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 3.191
R-factor	0.2552
Rwork	0.254
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	3.250
High resolution limit [Å]	3.018	3.018
Rmerge	0.160	0.861
Number of reflections	27684	1355
<I/σ(I)>	13.1	3.1
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100 mM Sodium Citrate pH 6.0, 200 mM Magnesium Acetate, 50 mM DTT, 14% PEG 8000