8QJP
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with uridine - 5'- monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-21 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.434, 72.800, 82.928 |
Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
Resolution | 43.410 - 1.200 |
R-factor | 0.1369 |
Rwork | 0.134 |
R-free | 0.16690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.560 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.410 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.081 | 1.088 |
Rmeas | 0.090 | 1.352 |
Number of reflections | 155893 | 7417 |
<I/σ(I)> | 11.5 | 1.4 |
Completeness [%] | 97.0 | 94 |
Redundancy | 5.1 | 5.1 |
CC(1/2) | 0.998 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES pH 6.5, 30 % w/v PEG 8000, 0.2 M Lithium sulfate. Protein concentration 11.8 mg/ml. Cryo-protection by glycerol 20 % v/v. |