8QJO
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with guanosine-5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.178, 73.068, 81.607 |
Unit cell angles | 90.00, 105.32, 90.00 |
Refinement procedure
Resolution | 41.640 - 1.850 |
R-factor | 0.1723 |
Rwork | 0.171 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.445 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.640 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.129 | 1.295 |
Rmeas | 0.152 | 1.523 |
Number of reflections | 40335 | 2553 |
<I/σ(I)> | 7.1 | 1.1 |
Completeness [%] | 96.4 | 99.3 |
Redundancy | 3.5 | |
CC(1/2) | 0.994 | 0.376 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M Lithium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350, protein concentration 7.5 mg/ml. Cryo-protection by glycerol 20 % v/v. |