8QJN

SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with adenosine-5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	44.470, 72.741, 82.752
Unit cell angles	90.00, 102.68, 90.00

Refinement procedure

Resolution	43.385 - 1.650
R-factor	0.1759
Rwork	0.175
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.569
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.385	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.105	0.869
Rmeas	0.113	0.935
Number of reflections	52701	2636
<I/σ(I)>	13.2	2.2
Completeness [%]	88.8	92.8
Redundancy	6.9
CC(1/2)	0.998	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M Ammonium sulfate 0.1 M MES pH 6.5, 30% w/v Polyethylene glycol 8000. Protein concentration 8.5 mg/ml. Cryo-protection by glycerol 20 % v/v.