8QJM
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with cytidine-5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-21 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.3418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.139, 72.805, 81.238 |
Unit cell angles | 90.00, 105.07, 90.00 |
Refinement procedure
Resolution | 41.660 - 1.650 |
R-factor | 0.1732 |
Rwork | 0.173 |
R-free | 0.19790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.607 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.660 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.124 | 0.596 |
Rmeas | 0.149 | 0.722 |
Number of reflections | 57878 | 2787 |
<I/σ(I)> | 6.8 | |
Completeness [%] | 99.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.961 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M Lithium sulfate 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350. Protein concentration 7.5 mg/ml. Cryo-protection by PEG400 30 % v/v. |