8QJL

SmNuc1 nuclease from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	Bruker PHOTON II
Wavelength(s)	1.3418
Spacegroup name	P 21 21 2
Unit cell lengths	72.470, 72.970, 49.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.220 - 1.400
R-factor	0.1228
Rwork	0.119
R-free	0.16120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.692
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.220	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.118	1.166
Rmeas	0.122	1.320
Number of reflections	50815	2541
<I/σ(I)>	19.9
Completeness [%]	96.0	98.1
Redundancy	14.3	4.3
CC(1/2)	0.999	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M Lithium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350. Protein concentration 7.5 mg/ml. Cryo-protection by glycerol 20 % v/v.